OxSens

OxSens™ delivers high-end research reagents with performance comparable to top international products, filling a critical gap in the domestic market. The OxSens™ series is empowering industry partners and research institutions to achieve more efficient and precise biological science research.

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OxSens Ultra-low Input RNA Extraction & Library Prep Kit

This Kit is designed for high-throughput sequencing platforms (e.g., Illumina and MGI), offering a picogram-level library preparation solution. It is suitable for extracting RNA and constructing sequencing libraries from high-quality, partially degraded, or low-quality trace samples (e.g., serum, plasma, saliva, urine, bronchoalveolar lavage fluid, FFPE), as well as specific substances like cell-free nucleic acids, exosomes, and pathogenic microorganisms. The kit includes all reagents necessary for RNA extraction, fragmentation, reverse transcription, double-stranded cDNA synthesis, rRNA depletion, and library amplification.





$ 560 / 6T

Product Details Instructions
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OxSens Circulating RNA Complete BCT

This BCT is compatible with a variety of downstream applications. Cell-free RNA (cfRNA) and extracellular vesicles isolated from plasma can be used for qualitative and quantitative real-time PCR, Droplet Digital PCR, next-generation sequencing, and nanoparticle tracking analysis. This product is fully compatible with the OxSens™ Ultra-low Input RNA Extraction & Library Prep Kit.





$ 56 / box

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OxSens iPSC Culture Medium

Induced pluripotent stem cells (iPSCs) possess the ability to differentiate into ectoderm, mesoderm, and endoderm, making them an ideal tool for stem cell research, disease modeling, and drug development. The OxSens iPSC Culture Medium is specifically designed for feeder-free culturing of human iPSCs, eliminating animal-derived components to significantly reduce contamination risks while ensuring consistency and reproducibility in cell culture. In comparative tests with mainstream media like E8 and mTeSR, the OxSens iPSC Culture Medium demonstrated performance consistent with mTeSR after multiple passages.




$ 420 / 500 ml

Product Details Instructions

FAQ

If degradation occurs during extraction, the following reasons may apply:
1. Tissue material not fresh: Use fresh tissue material, or rapidly freeze fresh tissue in liquid nitrogen and store at -80°C.
2. Contamination with RNases in reagents or equipment: Before starting the experiment, refer to "Precautions for Preventing RNase Contamination" to avoid RNase contamination.
3. High RNase content in the tissue material: For tissues or cells with high RNase activity, reduce the starting sample amount and increase the volume of Buffer RL.  

If the RNA Spin Column becomes clogged while using this kit, the following reasons may apply:
1. Incomplete sample lysis: Insufficient lysis of the sample can lead to clogging of the RNA Spin Column. For tissue or cell lysis, refer to the "Tissue or Cell Lysis" section in the protocol. For difficult-to-lyse tissues, it is recommended to use liquid nitrogen grinding for lysis.
2. Excessive starting sample amount: Too much sample can result in an overload of nucleic acids, causing clogging. For the optimal starting amount of tissue or cells when using this kit, refer to "Optimal Starting Amounts and Buffer RL Usage for Different Tissues."
3. Centrifugation temperature too low: Unless otherwise specified, RNA extraction with this kit should be performed at room temperature (20–25°C). Temperatures that are too high or too low can affect the RNA Spin Column and lead to clogging.  

For expected RNA yields from tissues or cells extracted with this kit, refer to "RNA Yields from Different Tissues." If the RNA yield is too low, the following reasons may apply:
1. Incomplete sample lysis: Thorough lysis of the sample is critical for extracting high-quality RNA with this kit. For sample lysis, refer to the "Tissue or Cell Lysis" section in the protocol.
2. Excessive starting sample amount: For the optimal starting amount of tissue or cells, refer to "Optimal Starting Amounts and Buffer RL Usage for Different Tissues."
3. Incomplete RNA elution: It is recommended to perform a second elution step. During elution, extend the incubation time of RNase-Free dH₂O or 0.1% DEPC-treated dH₂O on the RNA Spin Column to 10 minutes.
4. Ethanol residue in the eluate: If the RNA Spin Column is not centrifuged after washing with Buffer RWB, residual ethanol may remain, reducing RNA yield. It is recommended to perform an additional centrifugation step after washing with Buffer RWB to remove residual ethanol. 

Sodium citrate.

The collection tubes come in 2 ml and 5 ml models, with the blood volume automatically regulated and controlled by negative pressure. Unless there are specific needs, a 2 ml collection is recommended.   

A 5 ml centrifuge can be used, or the sample can be transferred to a 2 ml centrifuge tube. The tube rack is compatible with a 15.5 mm diameter.

The basal medium should be stored at -20°C, and the additives should be frozen at -80°C to -20°C. Once mixed, the complete medium should be refrigerated at 2–8°C and used within 2 weeks.  

The culture medium has been validated to support iPSC passaging for up to 50 generations while maintaining self-renewal capacity and an undifferentiated state without changes. 

Follow aseptic techniques during use. After opening, use the medium as soon as possible to avoid repeated freeze-thaw cycles, contamination, or degradation.  

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